Preparation and spectroscopic determination of C phycocyanin, R phycoglobin and B phycoglobin by salting out

Abstract

Phycobilin is an important pigment protein in photosynthesis, which mainly exists in blue-green algae. According to their maximum absorption peaks in the spectrum, they can be divided into C-phycocyanin, R-phycoglobin and B-phycoglobin. These three phycobilin proteins each have specific functions in living organisms, and their existence and mechanism of action in nature are also different. In this paper, we describe in detail the preparation process of C-phycocyanin, R-phycoglobin and B-phycoglobin, and how to determine them by spectroscopic method to understand their spectral characteristics. First, the cells are broken and then coarse extracted using a salt-out extraction method. The physical, chemical and spectral characteristics of three phycobilins were studied by various analytical methods, including UV-VIS spectroscopy, fluorescence spectroscopy and circular binary chromatography.